HR-LCMS based Metabolites Profiling of Madhuca Longifolia (J.Konig) J. F. Macbr. Flower Extract and its Bioactivity

Digambar Subhashrao Pawar, Jyoti Udhavrao Ghodke

Abstract: Madhuca longifolia, commonly known as mahua is a very important medicinal plant belongs to family sapotaceae. It is large size tree frequently occurred in India and Indian sub continent. Their flowers are used as food, fermented and non fermented products among the tribal people. It is also used as medicine to cure various ailments, due to presence of secondary metabolites [14]. So in this present study, we investigate its methanolic extracts of flowers for bioactive compounds by HR-LCMS and antibacterial properties by using disc diffusion method against plant pathogenic bacteria, such as Xanthomonus axonopodis and Pseudomonas syringae. Plant extract were showed antibacterial activity against both plant pathogenic bacteria. The maximum growth of inhibition were recorded against X. axonopodis (16 mm) followed by P. syringae (15 mm). Due to the presence of some important bioactive compounds such as Mebeverine metabolite, Quercitrin, Dihydrodeoxystreptomycin, Leucine, Dihydromyricetin, Protorifamycin-I, Chlortetracycline, Racepinephrine, asparatic acid, 4-Trimethylammoniobutanal, arginine, Glutamic acid, Salicyl acyl glucuronide, Petunidin, Betaxolol (hydroxylation), 16-hydroxy-5-hexadecenoic acid, Coproporphyrin-II, Dimethyl sulfone, etc. Our findings provided facts that methanolic extract of these tested plants have medicinally significant bioactive compounds and it justifies their use in the traditional medicines for the dealing of various diseases.

Keywords: HR-LCMS, Xanthomonus axonopodis, Pseudomonas syringae and Madhuca longifolia.

How to Cite?: Digambar Subhashrao Pawar, Jyoti Udhavrao Ghodke, "HR-LCMS based Metabolites Profiling of Madhuca Longifolia (J.Konig) J. F. Macbr. Flower Extract and its Bioactivity", Volume 14 Issue 8, August 2025, International Journal of Science and Research (IJSR), Pages: 1067-1072, https://www.ijsr.net/getabstract.php?paperid=SR25817154348, DOI: https://dx.doi.org/10.21275/SR25817154348