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Experimental Result Paper | Biological Sciences | India | Volume 12 Issue 4, April 2023
Anti-bacterial, Anti-Inflammatory Activity and Bio-Active Compounds Analysis of Curvularia lunata an Endophytic Fungi Isolated from Justicia Tranquebariensis-Using GCMS
K. Usharani | K. Usharani
Abstract: Medicinal plants are the local heritage with universal importance. World was gifted with a rich wealth of medicinal plants. Medicinal plants, as source of remedies, are widely used as alternative therapeutic tools for the prevention or treatment of many diseases. Justiciatranquebariensis is a small shrub, which is widely distributed in southern parts of India. From this plant fungal endophytes were isolated on PDA medium and identified as Curvularialunata. The fungus was cultured on potato dextrose broth for production of fungal metabolites. Ethyl acetate extract of the C. lunata was obtained by liquid-liquid partition of broth of endophyte and evaporation. Dried crude extract was tested for anti-bacterial activity by agar well diffusion method. The extract showed antibacterial nature against all the test pathogens (ATCC Cultures). Dried crude extract was also tested for anti-inflammatory activity by egg albumin denaturation method, ability of the extract to inhibit protein denaturation was studied. Ethanolic extract of C. lunata was elucidated using Gas Chromatography-Mass Spectrometry method. Twenty compounds were identified. Among these 20, nearly 12 Compounds are reported as anti-inflammatory principles. From this study, it is obvious that Curvularialunata an Endophytic fungi isolated from Justiciatranquebariensis extracts contain many biologically active compounds, Such as Antioxidant, Anticancer, Antitumor, Antimicrobial, and Anti-inflammatory. Hence further studies are required to isolate and purify the compound to assess the nature of the compound and its mode of action to take this study in to the next level of research in the future.
Keywords: Endophytic fungi, agar well diffusion, bioactivecompounds, GCMS Analysis, Tetracosamethyl-cyclododecasiloxane, Anti-inflammatory activity
Edition: Volume 12 Issue 4, April 2023,
Pages: 1423 - 1427