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Research Paper | Microbiology | India | Volume 4 Issue 1, January 2015
Production of Indole Acetic Acid (IAA) by Stenotrophomonas Maltophilia BE25 Isolated from Roots of Banana (musa spp)
M. S. Ambawade [2] | G.R. Pathade
Abstract: In the present study based on morphological, cultural, biochemical and 16s rRNA gene sequencing a newly isolated endophytic bacterium from the root of the banana (musa spp) was identified as Stenotrophomonas maltophilia and was designated as BE 25. Twenty two isolates were screened out for their productivity of indole acetic acid (IAA) by salkowskis method on spectrophotometer. Out of these isolates BE-25 isolate showed high amount of indole acetic acid production supplemented with and without L-tryptophan in medium. IAA production of BE-25 was further confirmed by thin-layer chromatography (Rf-0.54cm) as well as by high-performance liquid chromatography (38.85, 39.72ppm at 280 nm) with and without L-tryptophan in medium.
Keywords: Banana, 16s rRNA gene sequencing, IAA, HPLC
Edition: Volume 4 Issue 1, January 2015,
Pages: 2644 - 2650