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Research Paper | Biotechnology | Volume 15 Issue 2, February 2026 | Pages: 1000 - 1006 | India
Enhanced FK-506 Production by a New Strain Streptomyces Glaucescens SS-13 through Ultraviolet Irradiation-Induced Mutagenesis
Abstract: Tacrolimus (FK-506) is a significant polyketide produced by Streptomyces sp. It is considered the most important immunosuppressant molecule, and its estimated market size in 2030 for tacrolimus is around US$10 billion. The lower productivity of the product during the fermentation causes major challenges from an industrial perspective. In this study, we performed classical UV mutagenesis to enhance the production of Tacrolimus (FK-506) by a newly reported strain, Streptomyces glaucescens MTCC 276. The wild-type strain was a lower producer of FK-506 and lacked industrial importance. We have screened several mutant strains to identify a potent isolate with higher productivity. The results indicated that the screening of the mutant strain S. glaucescens, SS-13, led to a maximum tacrolimus production of approximately 125 mg/L. We also investigated the effect of the surfactant Tween-20 to improve tacrolimus production. Additionally, our findings on the effect of Tween-20 revealed that an optimal concentration of 10 g/L resulted in a higher yield of around 96 mg/L. Therefore, further chemical mutagenesis and fermentation studies can be conducted with this potent mutant strain to maximize its titer value. The SS-13 mutant strain shows promise for efficient and significant industrial application in the future. The growth parameters, such as pH, temperature, and nutrient feeding, were also studied to enhance the production of FK-506.
Keywords: Tacrolimus, Streptomyces glaucescens, UV mutagenesis, Fermentation, Tween-20
How to Cite?: Parvathi S., Saravanan J., "Enhanced FK-506 Production by a New Strain Streptomyces Glaucescens SS-13 through Ultraviolet Irradiation-Induced Mutagenesis", Volume 15 Issue 2, February 2026, International Journal of Science and Research (IJSR), Pages: 1000-1006, https://www.ijsr.net/getabstract.php?paperid=SR26216164117, DOI: https://dx.dx.doi.org/10.21275/SR26216164117