Algal Biofuel: Modified Tag Production

Joseph Rop, Shawn Kraus

Abstract: Algal biofuel today presents some major limitations which keep it from being used as a primary energy source globally. The two major methods of production are: open biofuel systems and closed bio photoreactors. These present two extremes of algae biofuel limitations in the present day. While external factors are what limit these forms of production, internal factors must not be ignored. This research project focuses on the development of a method for altering the genetic component found in the algae species chlorella vulgaris to increase its biofuel productivity rates. The process will comprise 2 important processes: the selection of an appropriate genome which, if changed, could increase productivity, as well as the use of the genome editing tool, CRISPR/Cas9 to replace this genome. This design portfolio will include the following: costs of development, process of documentation, and constraints/limitations. To help with refining the biofuel reactor model it should be assessed in industry to determine its commercial application/viability.

Keywords: Biofuel, tag production, CRISPR, Cas9, triacylglycerol (TAG), G3DPH, plasmid

How to Cite?: Joseph Rop, Shawn Kraus, "Algal Biofuel: Modified Tag Production", Volume 14 Issue 8, August 2025, International Journal of Science and Research (IJSR), Pages: 263-269, https://www.ijsr.net/getabstract.php?paperid=SR25723021400, DOI: https://dx.doi.org/10.21275/SR25723021400