Study of Antibacterial and Antifungal Potential of Leaf Extract of Xanthium Strumarium

Mohanish V. Wankhade

Abstract: For the study of antibacterial and antifungal activities, leaf extract of xanthium was prepared in 100% aceton. Three bacteria, Staphylococcus aureus (ATCC No. 6838), Bacillus subtilis (ATCC No. 6633), and Pseudomonas aeruginosa (ATCC No. 15442), as well as two fungal strains, Candida albicans (ATCC No. 14053) and Aspergillus niger (ATCC No. 11414), were the subjects of an antimicrobial study using varying concentrations of Xanthium leaf extract (5 mg and 10 mg). The agar well plate method was used to determine the antimicrobial screening. The antimicrobial activity is estimated by comparing the zone of inhibition of growth of sensitive micro-organisms produced by known concentration of the isolated substance or extract or synthetic compound to be examined against a reference substance. Among the tested bacteria strains, Bacillus subtilis was the most sensitive to leaf extract of Xanthium while S.aureus and P. aeruginosa showed the least response. While studying the antifungal effect indicate that leaf extract of Xanthium may have moderate efficacy.

Keywords: Antibacterial, Antifungal, Xanthium, ATCC, acetone, Zone of Inhibition