Effects of Justicia Adhatoda L. and Clerodendrum wallichi Merr. Crude Leaves Extracts on Protein Denaturation Inhibition for Potential Anti - Inflammatory Activity: In Vitro

Elareen Belljoy Donshiew, Surya Bhan

Abstract: The current study aimed to investigate the anti - inflammatory activity of methanolic and butanolic leaves extract of Justicia adhatoda L. butanolic extract (JABE), methanolic extract (JAME) and Clerodendrum wallichi Merr. Butanolic extract (CWBE), methanolic extract (CWME). The anti - inflammatory activity of these extracts was evaluated using in vitro - based assays: protein denaturation inhibition. Methanolic and butanolic extracts exhibited a concentration dependent inhibition of protein (egg albumin and BSA). Results showed that the percentage inhibition of protein denaturation at concentration of 500?g/ml of JABE has exhibited a significantly higher inhibition of 61.65% in egg and 81.21% in BSA, CWBE showed 80.33% in egg and 73.02% in BSA. Also, JAME exhibit a significant increase in protein inhibition of 59.69% in egg and 76.89% in BSA, CWME 76.1% in egg and 71.44% in BSA. In conclusion, results revealed that the studied leaves possess anti - inflammatory properties at different levels, and this could be due to the differences in the composition and concentration of bioactive compounds. The objective of the present study is to ameliorate in vitro conformation of potential inhibition of protein denaturation by using the methanolic and butanolic extracts of Justicia adhatoda L. and Clerodendrum wallichi Mer. The present study put forward that the crude extract of Justicia adhatoda L. and Clerodendrum wallichi Mer. act as potent in vitro anti - inflammatory activity.

Keywords: Medicinal plant, anti-inflammatory activity, Egg albumin, denaturation, inhibition, Bovine serum albumin, BSA