Seasonal Variations in Primary Metabolites and their Statistical Analysis in Trigonella foenum-graecum

Dr. Vasantrao B. Kadam

Abstract: The present study investigated the seasonal variation in primary metabolites of Trigonella foenum-graecum (fenugreek), an important medicinal and culinary plant. Hypothetical yet scientifically realistic data were generated for three seasonal conditions: Winter, Summer, and Monsoon. Four primary metabolites-total carbohydrates, total proteins, total lipids, and free amino acids-were quantified using standard biochemical protocols. Five biological replicates were considered per season. Statistical analyses included mean ? standard deviation, one-way ANOVA, and pairwise t-tests with Bonferroni correction. The results revealed significant seasonal fluctuations (p < 0.05) in metabolite levels, with the highest concentrations of carbohydrates and proteins observed during winter and monsoon, respectively. Lipid content showed moderate seasonal variation, while free amino acids peaked in monsoon samples. The findings suggest that seasonal factors may influence metabolic composition and, consequently, the nutritional and therapeutic value of T. foenum-graecum. These insights can aid in optimizing harvesting periods for nutraceutical and pharmacological applications.

Keywords: Trigonella foenum-graecum, seasonal variation, primary metabolites, statistical analysis, ANOVA, carbohydrates, proteins, lipids, amino acids

How to Cite?: Dr. Vasantrao B. Kadam, "Seasonal Variations in Primary Metabolites and their Statistical Analysis in Trigonella foenum-graecum", Volume 14 Issue 12, December 2025, International Journal of Science and Research (IJSR), Pages: 1600-1602, https://www.ijsr.net/getabstract.php?paperid=SR251219130644, DOI: https://dx.doi.org/10.21275/SR251219130644