Isolation and Characterization of Biosurfactant from Klebsiella pneumoniae subsp.rhinoscleromatis

Ashwini Thorat, Vedanti Dikey, Sakshi Deshpande, Rohini Pungle

Abstract: Biosurfactant is surface active compounds produced by microorganisms. Oil contaminated soil sample were collected from motor garage and microorganisms were screened for biosurfactant production using enrichment culture techniques. Various screening methods, including emulsification index, drop collapse method, CTAB agar method, were applied to identify the most efficient biosurfactant producer. S4.1 was found to be maximum producer of biosurfactant which has been identified as Klebsiella Pneumoniae subsp.rhinoscleromatis by 16S rRNA sequencing. Biosurfactant production by S4.1was optimum at incubation period of 96 hr., pH 5 and 1% glucose as carbon source. Cell free extract consisting biosurfactant has maximum activity at acidic and alkaline conditions and at temperature 30?C. The oil spreading method and hemolytic activity confirmed presence of biosurfactant in extracted and purified biosurfactant. The thin layer chromatography (TLC) and fourier transform infrared spectroscopy (FTIR) confirmed the purified biosurfactant as glycolipoprotein. Biosurfactant was found to have antimicrobial activity against E.coli and Pseudomonas spp. and had ability to form micelle.

Keywords: Biosurfactant, emulsification index, drop collapse method, CTAB agar, Klebsiella pneumoniae subsp.rhinoscleromatis

How to Cite?: Ashwini Thorat, Vedanti Dikey, Sakshi Deshpande, Rohini Pungle, "Isolation and Characterization of Biosurfactant from Klebsiella pneumoniae subsp.rhinoscleromatis", Volume 14 Issue 11, November 2025, International Journal of Science and Research (IJSR), Pages: 1880-1889, https://www.ijsr.net/getabstract.php?paperid=SR251127194846, DOI: https://dx.doi.org/10.21275/SR251127194846