Alcohol Oxidase Linked Enzyme Cascade as Anodic Biofuel Cell Catalyst

Madhuri Das

Abstract: This study explores the development of a biofuel cell utilizing alcohol oxidase AOx in an enzyme cascade at the anode. The aim was to increase power density by replacing NAD based dehydrogenases with AOx for the oxidation of methanol. The bioanode with AOx, coupled with formaldehyde and formate dehydrogenases, achieved enhanced power density compared to conventional systems. The electrochemical characterization of the bioelectrode demonstrated effective redox behavior and stability over time. This biofuel cell system represents a step toward more efficient energy production from alcohol substrates.

Keywords: Alcohol oxidase, biofuel cell, enzyme cascade, methanol oxidation, NAD+ regeneration

How to Cite?: Madhuri Das, "Alcohol Oxidase Linked Enzyme Cascade as Anodic Biofuel Cell Catalyst", Volume 13 Issue 9, September 2024, International Journal of Science and Research (IJSR), Pages: 1121-1127, https://www.ijsr.net/getabstract.php?paperid=SR24919110134, DOI: https://dx.doi.org/10.21275/SR24919110134