Microdeletion Screening of Azoospermia Factor d (AZFd) at Y Chromosome

Krunal Chudasama, Mansi Dadhania, Dr. Jenabhai B. Chauhan

Abstract: Aim: To determine the frequency of Yq chromosome microdeletions in infertile men from Gujarat. Introduction: Male infertility, contributing to 9% of global infertility cases, is often linked to Y-chromosome microdeletions, especially in the AZF region. Methods: Fifty-eight male infertility samples (40 normozoospermic, 13 oligozoospermic, 5 azoospermic) were analyzed. Sperm morphology was assessed via Papanicolaou staining. DNA was extracted using the Phenol-Chloroform method, and PCR detected Yq microdeletions using sY152 and SRY sY14 markers. Results: Among the samples, 67.24% had reduced motility, 79.31% exhibited >14% morphological defects, and 10.34% showed AZFd microdeletions. Conclusion: Sperm morphological defects and Yq microdeletions contribute to infertility. Y-chromosome deletion testing has diagnostic and prognostic significance, aiding screening for IVF clinics.

Keywords: Microdeletions, Azoospermia factor (AZF), Y chromosome, Deletion frequency, PCR analysis, IVF clinic