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Research Paper | Botany | India | Volume 13 Issue 1, January 2024
Phytochemical and Antibacterial Investigation of Stem Bark and Callus Extracts of Holarrhenaantidysenterica Wall
Suchitra Mahato | Anita Mehta [4]
Abstract: In the present investigation, the methanolic extracts of bark and callus of Holarrhenaantidysenterica were evaluated for antimicrobial activity against common human pathogens and subsequently phytochemical analysis of the crude extracts was carried out to determine the active phytochemical constituents responsible for antimicrobial activity. Explant used for callus formation were mainly seeds and in vivo and in vitro grown plant parts like stem, nodal parts, roots and apical shoots. A friable type of callus was obtained when explants cultured on MS medium with 2, 4 - D separately or in combination of KN. This study also involves the bioseparation of compounds present in the crude extracts of bark and callus by TLC and HPTLC methods. Presence of peaks in the TLC and HPTLC exhibited presence of principle bioactive compound conessine in the methanolic extracts of Bark and Callus. It was observed that methanolic bark extract of Holarrhenaantidysenterica was more effective in inhibiting all the two tested pathogens with zone of inhibition ranging between 2.3 mm to 10.05 mm as compared to methanolic callus extract with 1.65 mm to 4.0 mm of inhibition zone only. Phytochemical profiling of crude bark and callus extracts revealed that both the extract contain alkaloids, tannins, steroids and flavonoids. The results indicate that the methanolic bark and callus extracts might be exploited as natural drug for the treatment of several infectious diseases caused by these organisms.
Keywords: Holarrhena antiydsenterica. Methanolic extracts, antimicrobial activity, phytochemical, HPTLC
Edition: Volume 13 Issue 1, January 2024,
Pages: 757 - 762