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Research Paper | Biochemistry | India | Volume 13 Issue 1, January 2024

Structural and Biophysical Studies of ?-Galactosidase from Oryza sativa (Osbg)

Soha Aggarwal

Abstract: Beta-galactosidase has a very special space in the history of molecular biology. It proved to be a strong basis for the development of the Jacob and Monod model for gene expression. Here in this work, the enzyme has been isolated from Oryza sativa. A simple protocol for extraction and purification of the enzyme was chosen. Anion-exchange chromatography was performed to purify the enzyme. Circular Dichroism gave an important information about the ?-helix (15.9%) and ?-strands (30.4%) in the enzyme. In-silico methods have been implemented to attain the three-dimensional structure of ?-galactosidase from Oryza sativa. To study the catalytic sites molecular docking have been performed. This work supports the structure-based drug designing for developing an analogue in place of non-functional lactase enzymes.

Keywords: beta-galactosidase, gene, molecular biology, molecular docking

Edition: Volume 13 Issue 1, January 2024,

Pages: 337 - 341