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Research Paper | Biotechnology | India | Volume 12 Issue 12, December 2023
Cost Effective Production of Bacillus thuringiensis israelensis using Rotten Pineapples
Dr. Gopinathan C | Devika M Shaju | Hana Mol K E
Abstract: Vector-borne ailments are illnesses that are transmitted by way of vectors. These vectors can elevate infective pathogens such as viruses, bacteria and protozoa from one host to another. For the prevention of these diseases, vector management could be a powerful preventive tool. Use of biopesticide is safer than use of chemicals or chemical derived pesticides posing low risk to environment and humans. Microbial insecticide is a most extensively used biopesticide. Bacillus thuringiensis israelensis (Bti) serves as an ideal candidate for mosquito control due to the fact of its excessive mosquito larvicidal activity, environmental friendliness, safety to non-target organisms and mammals, ease of production and ability to withstand various formulations like a dust, pellets, sprays, briquettes, capsules, granules, etc. In the present study, using pineapple juice as substrate, effect of submerged fermentation, solid state fermentation, batch fermentation and fed batch fermentation on the cultivation of Bti was investigated. Compared with batch fermentation, fixed volume fed-batch fermentation showed increase in biomass by 23.8%. 80 % solid state fermentation showed maximum biomass among all the cultivation. Studies have also been conducted using cheap industrial substrate together with chicken manure, fish waste, urea and soybean powder as nitrogenous sources which will greatly help in cutting down production cost of Bti. Finally, biolarvicidal activity of the Btiwere also evaluated using Aedes aegypti. 70-98 % mortality was also observed in pineapple extract media supplemented with different nitrogenous wastes.
Keywords: Bacillus thuringiensis israelensis, Fed batch fermentation, Batch fermentation, Fixed volume fed-batch fermentation, Solid-state fermentation, Biopesticide
Edition: Volume 12 Issue 12, December 2023,
Pages: 1202 - 1208