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Research Paper | Zoology | India | Volume 12 Issue 7, July 2023
Dimethoate Mediated Some Behavioural, Hematological and Histopathological Changes in Channa Punctatus
Abstract: The present study investigated some behavioural, hematological and histopathological changes caused by sublethal concentration (LC50 value= 5.56 ?g/l) of dimethoate in Channa punctatus. No adverse behavioral and physiological changes were observed in the control group, but the pesticide exposed group exhibited erratic swimming, rapid opercula movement, and release of bubbles, frequent surfacing, loss of equilibrium (somersaulting) and sudden jerk movement. The RBC reduced significantly (p<0.05). WBC significantly (p<0.05) increased by 4.72% and 9.23% in test Groups C and D, respectively. Contrariwise, mean hemoglobin (Hb) values reduced significantly (p<0.05) by 5.11%, 19.23% and 28.24% in Groups B, C and D, respectively compared to the control. Mean corpuscular hemoglobin concentration (MCHC), mean corpuscular hemoglobin (MCH) and mean cell volume (MCV) followed the same pattern as Hb and PCV with the lowest values in the highest pesticide concentration. Exposure group showed a disorganization of intra-cardiac chamber structure, and saw-toothed intra-cardiac chamber muscles with loss of intra-cardiac muscle fiber. Damage to the cardiac muscles was worse in group D with focal loss of cardiac muscle and intra-cardiac hemorrhage. Spongiosis and infiltration of inflammatory cells (microglial cells) were observed in the brain of exposure group. In the gills, histological damages with distortion of gill epithelium, lifting of gill epithelium from the gill stock, disorganization and fragmentation of parts of the gill. Aggregation of inflammatory hepatocytes, and necrosis and distortion of hepatic architecture were observed in the pesticide exposed groups. The kidneys of the fish were exposed to different concentrations of the pesticide showed distortion of cell architecture, fragmentation, loss of renal tissues and intra-renal hemorrhages (bleeding in the renal cells). Histology of the fin of exposed fish showed fragmentation in the fin and separation from the attached muscles.
Keywords: Dimethoate pesticide, Channa punctatus, Hematology, Histopathology
Edition: Volume 12 Issue 7, July 2023,
Pages: 434 - 438