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Research Paper | Pathology | India | Volume 12 Issue 4, April 2023
To Study the Significance of Micronuclei and it?s Scoring in Effusion Fluids
Abstract: Background : Micronuclei are extra-nuclear bodies which contains fragments of damaged chromosomes and/or whole chromosome that were not incorporated into the nucleus during anaphase stage of cell division, as they lack centromere. MN formation is an important mechanism for chromosome loss in the nucleus.  Chromosomal instability leads to formation of MN which plays an important role in carcinogenesis.  MN scoring in effusions can be done to differentiate benign and malignant effusions. Aim of the study was to score MN in effusion fluids and to differentiate malignant effusions from benign effusions on the basis of MN score. Materials & Methods: A total 113 samples of ascitic and pleural effusions received for fluid cytology were studied. These fluid smears were stained with conventional papanicolaou stain. Number of micronuclei present per 1000 well preserved cells were counted under light microscopy. Result: Mean micronuclei score in malignant and benign ascitic effusions were 5.58 and 2.72 respectively, while in pleural effusions 4.0 and 1.8 respectively. Mann Whitney test showed that this difference was statistically signi?cant (P ? 0.001). Conclusion: Micronuclei scoring can be used as a biomarker of chromosomal instability in routine reporting in distinguishing between malignant and benign effusions. MN scoring may be a simple, easy, alternative diagnostic tool to distinguish between benign & malignant effusions in low resource setting.
Keywords: Fluid cytology, malignancy, micronuclei, score
Edition: Volume 12 Issue 4, April 2023,
Pages: 1050 - 1053