Assessment of Diagnostic Values and Comparative Evaluation of Bone Marrow Aspiration and Bone Marrow Clot Section in Various Hematological Disorders

Pendram S, Jaiswal K, Tijare J, Deshpande A

Abstract: Introduction: Bone marrow examination has become an indispensable adjuvant to diagnose the diseases of blood & other body system. Bone marrow examination included Bone marrow aspiration, clot section & Bone marrow biopsy. Aspiration & clot section if done simultaneously yield a good diagnostic material, which will be more accurate for diagnosis. Method: Under all aseptic precaution & local anaesthesia Bone marrow aspirate obtained from posterior superior iliac spine, 4 - 6 slides were made from Bone marrow aspirate & stained with Leishman & Giemsa. Remaining aspirate kept on slide to form clot. This clot fixed in 10% formalin & processed in histopathology as a routine tissue. Sections were made from clot & stained with Haematoxylin & Eosin & with special stained whenever required. Result: Here we present 3 cases. Case 1: 12 year female with complaints of acute onset of fever & breathlessness. Findings: CBC - Within normal limit. PS & Bone marrow aspiration - Infection related reactive cellular changes in Bone marrow (Hemophagocytosis) Bone marrow clot section on ZN staining confirmed diagnosis of Tuberculosis. Case 2: 44 year male with known case of Internal haemorrhoids with per rectal bleeding. PS & CBC: - Pancytopenia. Bone marrow aspiration: - Diluted marrow. Bone marrow clot section: - Increase in fat: cell ratio showing features of aplastic anaemia. Bone marrow biopsy was inadequate in this case. Showing only single trabeculae. Case 3: 55 year male with history of fever, weight loss and generalized weakness. PS & CBC: - Pancytopenia. Bone marrow aspiration: - Diluted marrow. Bone marrow clot section: - Grade 2 myelofibrosis, which was later confirmed on Bone marrow biopsy. Conclusion: A complementary technique (Bone marrow clot section) from bone marrow aspirate helps to rule out differential & early diagnosis.

Keywords: CBC, Complete blood count, PS, Peripheral smear, BMA, Bone marrow aspiration, BMBx, Bone marrow biopsy

How to Cite?: Pendram S, Jaiswal K, Tijare J, Deshpande A, "Assessment of Diagnostic Values and Comparative Evaluation of Bone Marrow Aspiration and Bone Marrow Clot Section in Various Hematological Disorders", Volume 12 Issue 4, April 2023, International Journal of Science and Research (IJSR), Pages: 95-98, https://www.ijsr.net/getabstract.php?paperid=SR23329010600, DOI: https://dx.doi.org/10.21275/SR23329010600