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Case Studies | Pathology | India | Volume 12 Issue 4, April 2023
Assessment of Diagnostic Values and Comparative Evaluation of Bone Marrow Aspiration and Bone Marrow Clot Section in Various Hematological Disorders
Pendram S | Jaiswal K | Tijare J | Deshpande A
Abstract: Introduction: Bone marrow examination has become an indispensable adjuvant to diagnose the diseases of blood & other body system. Bone marrow examination included Bone marrow aspiration, clot section & Bone marrow biopsy. Aspiration & clot section if done simultaneously yield a good diagnostic material, which will be more accurate for diagnosis. Method: Under all aseptic precaution & local anaesthesia Bone marrow aspirate obtained from posterior superior iliac spine, 4 - 6 slides were made from Bone marrow aspirate & stained with Leishman & Giemsa. Remaining aspirate kept on slide to form clot. This clot fixed in 10% formalin & processed in histopathology as a routine tissue. Sections were made from clot & stained with Haematoxylin & Eosin & with special stained whenever required. Result: Here we present 3 cases. Case 1: 12 year female with complaints of acute onset of fever & breathlessness. Findings: CBC - Within normal limit. PS & Bone marrow aspiration - Infection related reactive cellular changes in Bone marrow (Hemophagocytosis) Bone marrow clot section on ZN staining confirmed diagnosis of Tuberculosis. Case 2: 44 year male with known case of Internal haemorrhoids with per rectal bleeding. PS & CBC: - Pancytopenia. Bone marrow aspiration: - Diluted marrow. Bone marrow clot section: - Increase in fat: cell ratio showing features of aplastic anaemia. Bone marrow biopsy was inadequate in this case. Showing only single trabeculae. Case 3: 55 year male with history of fever, weight loss and generalized weakness. PS & CBC: - Pancytopenia. Bone marrow aspiration: - Diluted marrow. Bone marrow clot section: - Grade 2 myelofibrosis, which was later confirmed on Bone marrow biopsy. Conclusion: A complementary technique (Bone marrow clot section) from bone marrow aspirate helps to rule out differential & early diagnosis.
Keywords: CBC, Complete blood count, PS, Peripheral smear, BMA, Bone marrow aspiration, BMBx, Bone marrow biopsy
Edition: Volume 12 Issue 4, April 2023,
Pages: 95 - 98