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Research Paper | Agriculture | India | Volume 12 Issue 11, November 2023
Iron Toxicity, Tolerance, and Quantitative Trait Loci Mapping in Rice (Oryza sativa L.)
Amrita Giri | Ritu R Saxena | Anurag Soni | S S Porte | Sunil K Verma | Ravi R Saxena | S B Verulkar
Abstract: Background: An experiment was conducted in the rainout shelter comprising of five varieties/ genotypes of Indica rice, exposed under different concentrations of two different forms of iron viz., ferrous (FeSO4) and ferric (FeCl3). Visual scoring scale was used to screen the genotypes and effects of excess iron were examined on different vegetative traits in which root weight and shoot weight were found to be more sensitive to excess iron concentration of both forms andeffect of iron on different genotype examined. Main body: In this experiment, five varieties/ genotypes of Indica rice, exposed under different concentrations of two different forms of iron viz., ferrous (FeSO4) and ferric (FeCl3).In two different forms of iron, ferrous form was found to be toxic than ferric form but high amount of ferric chloride without chelating agent can be more toxic in hydroponics condition. On the basis of visual scoring, we identified 4 genotypes tolerant (Dagad Deshi, IBD-1, RRF 127, and RRF 105) and Swarna as susceptible genotype for both form ferrous and ferric iron. Cross of Swarna and IBD-1 was used for development of F4 generation and QTLs were identified on the basis of genotypic and phenotypic data obtained from F4 generation. A total of thirteen QTLs have been identified using interval mapping (IM) approach. These QTLs are major and minor QTLs based on R2 or phenotypic variance explained (PVE %). In composite interval mapping approach, a total of twenty-four major and minor QTLs were detected, of these QTLs, ten were the major QTLs. RM 152 and RM 264 markers on chromosome number 8 are highly significantly (PVE>10) associated with the variation of two traits shoot length and Fe+3content in shoot. Conclusion: Significant differences among genotypes for various traits associated with iron tolerance under different doses of iron. In general, high dose of iron have toxic effect on genotypes. In sources of iron ferrous form of iron was noticed as more toxic form but high amount of ferric iron without chelating agent become more toxic than ferrous. Root weight and shoot weight were found to be more sensitive to excess iron
Keywords: Rice, iron toxicity, tolerance, iron concentration, QTLs
Edition: Volume 12 Issue 11, November 2023,
Pages: 918 - 928