International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
Call for Papers | Fully Refereed | Open Access | Double Blind Peer Reviewed

ISSN: 2319-7064


Downloads: 5 | Views: 254 | Weekly Hits: ⮙3 | Monthly Hits: ⮙3

Research Paper | Histopathology | Saudi Arabia | Volume 11 Issue 5, May 2022 | Popularity: 5.6 / 10


     

Comparative Study of Conventional Staining Techniques in Cytology

Faris Merghani A Elmahdi, Thabat Ibrahim Alrfaie, Ehdaa Mahmmoud Alsubaihi, Bayan Abdurahman Alhakami, Ghazal Mahmoud Shalabi, Bushra Anwar Kurdi, Abrar Khalid Aloufi, Saad Ali S. Aljohani


Abstract: Background: Exfoliative cytology is the histopathologic examination of cells that have been obtained by their physical removal, followed by their placement on a glass slide, and then appropriately stained [1].The aim of this study was to compare the staining of cytologic Papanicolaou stain, hematoxylin andeosin stain, and may-grunwald-giemsa stain in oral mucosa smear. Materials and Methods: A total of 150 Cytological oral smears were taken from 50 volunteers using a sterile wooden tongue depressor. In each case, the surface epithelium of the buccal mucosa was scraped and applied to a clean frosted glass slide. The scraped cells were placed onto pre-cleaned slides. three slides were made from each subject. One was air-dried and stained with the (MGG) stain, while the other was wet fixed and stained with PAP and H&E. Results: The nuclear structure was excellent stained with Pap and H&E and was poor with MMG representing 88%, 80, and 5% respectively while the cytoplasm and background were excellent stained with Giemsa representing 80% and 60% and respectively. Conclusion: Pap-stained smears were better than H&E and Grunwald- Giemsa stain for oral cytology.


Keywords: Comparative, Staining Techniques, Cytology


Edition: Volume 11 Issue 5, May 2022


Pages: 876 - 879


DOI: https://www.doi.org/10.21275/SR22514114506



Make Sure to Disable the Pop-Up Blocker of Web Browser




Text copied to Clipboard!
Faris Merghani A Elmahdi, Thabat Ibrahim Alrfaie, Ehdaa Mahmmoud Alsubaihi, Bayan Abdurahman Alhakami, Ghazal Mahmoud Shalabi, Bushra Anwar Kurdi, Abrar Khalid Aloufi, Saad Ali S. Aljohani, "Comparative Study of Conventional Staining Techniques in Cytology", International Journal of Science and Research (IJSR), Volume 11 Issue 5, May 2022, pp. 876-879, https://www.ijsr.net/getabstract.php?paperid=SR22514114506, DOI: https://www.doi.org/10.21275/SR22514114506



Similar Articles

Downloads: 1

Research Paper, Histopathology, India, Volume 10 Issue 11, November 2021

Pages: 399 - 403

Implementing the Paris System into Reclassifying Urine Cytology: A Descriptive Analysis

Dr. Loretta Ann Pereira, Dr. Hilda Fernandes, Dr. Amritha Chidambaram

Share this Article

Downloads: 2 | Weekly Hits: ⮙1 | Monthly Hits: ⮙2

Research Paper, Histopathology, India, Volume 11 Issue 3, March 2022

Pages: 1369 - 1380

Histopathological Study of Lesions of Sino-Nasal Tract and Nasopharynx

Adimulam Hareesh Kumar, Vudata Ramya Swathi, M. Janaki

Share this Article

Downloads: 3 | Weekly Hits: ⮙3 | Monthly Hits: ⮙3

Research Paper, Histopathology, Saudi Arabia, Volume 13 Issue 7, July 2024

Pages: 952 - 957

Identification of Mucopolysaccharide Types in Diabetic Placenta and Umbilical Cord Tissues

Faris M. Elmahdi, Lobna E Ali, Sahar E. Osman, Hala G. Alssied, Lina A. Salim

Share this Article

Downloads: 14 | Weekly Hits: ⮙1 | Monthly Hits: ⮙2

Research Paper, Histopathology, Saudi Arabia, Volume 13 Issue 4, April 2024

Pages: 1058 - 1062

Detection of Micronuclei in Oral Mucosa among Saudi Smokers Using Papanicolaou Stain

Faris M. Elmahdi, Amina J. Alshinqiti, Jori O. Kareembakhsh, Hanadi A. Alahmadi, Abdullah A. Bakkari, Raghad A. Murad, Sara M. Altom

Share this Article

Downloads: 105

Case Studies, Histopathology, India, Volume 5 Issue 7, July 2016

Pages: 2148 - 2150

Saccrococcygeal Chondroid Chordoma: FNAC Diagnosis: A Case Report with Histological and Radiological Correlation

Dr. Mukta Meel, Dr. Mukesh Kumar Bhaskar

Share this Article



Top