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Doctoral Thesis | Medical Microbiology | India | Volume 11 Issue 12, December 2022
Detection of Carbapenemase Producing E. Coli by Phenotypic Method (Modified Carba NP Test)
Dr. Shama Tomar | Dr. Shiv Prasad Reddy B. | Dr. Sapna Chauhan | Ravi Vashistha
Abstract: Objective: Carbapenems are important antibiotics for multidrug - resistant Enterobacteriaceae. Resistance to carbapenem is increasing at an alarming rate worldwide leading to major therapeutic failures and increased mortality rate. Easy and effective detection of carbapenemase producing carbapenem - resistant Enterobacteriaceae the key to control dissemination of carbapenem resistance in community - acquired infection. The aim of present study was to evaluate efficacy of Modified Carba NP (CNP) for early detection of carbapenemase producing E. coli. Material and Methods: Study was performed in the Microbiology Department in Muzaffarnagar medical college, Muzaffarnagar. In this study clinical specimens like pus, blood, sputum, throat swab, endotracheal tube aspirate, CSF, urine, body fluids, etc. obtained from various ICU & OPD and emergencyA total of 95 CRE were subjected to Modified CNP test for the detection of CPE. Statistical Analysis: It was done on Statistical Package for the Social Sciences (SPSS) software, Results: A total of 95 CRE isolates were included in this study. Majority of CRE was isolated from age group 20 to 30 years (33%) followed by 41 to 60 years (20%) with male preponderance over female patients. Maximum number of CRE were isolated from samples received from wards 50 (48%) followed by intensive care unit (ICU) 27 (25%) and outdoor units 20 (19%). CRE was isolated from urine samples also 25 (27%). Out of 95 CRE isolates, 46 (43%) isolates were phenotypically were confirmed by Modified CNP test. Conclusion: Modified strip CNP test is simple and inexpensive test which is easy to perform and interpret and gives rapid results in less than 5 minutes. It has high degree of sensitivity and specificity. Modified strip CNP test shows significantly higher detection capacity for carbapenemase producers.
Keywords: Carbapenem, resistant, E. coli, Carbapenemase producing E. coli, Detection by phenotypic method, Modified Carba NP test (CNP), Carbapenemase producing Enterobacteriaceae
Edition: Volume 11 Issue 12, December 2022,
Pages: 582 - 584