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Research Paper | Natural Science | Philippines | Volume 9 Issue 5, May 2020

Reduction of Bacillus cereus sensulato Biofilms on Stainless Steel Surfaces by Pestalotiopsis sp. Culture Crude Extract

Rener S. De Jesus | Gina R. Dedeles

Abstract: The biofilm formation of fourBacillus cereus sensulato isolates was induced on milk pre-soiled stainless steel coupons (20 5 mm) under nutrient-depleted broth medium for a total of 48 h. Biofilms on these coupons were immersed for 15 min in 25, 50, or 100 mg/mL of Pestalotiopsis sp. (P-03) culture crude extract at 25 or 50C. Results showed that the biofilm cells were significantly reduced (p < 0.05) as compare to nontreated biofilms after immersion in 50 and 100 mg/mL of P-03 crude extract, which the most significant was found at 100 mg/mL with 2-Log reduction (99 %). The crude secondary metabolites obtained from P-03 fermentation broth cultures were detected through thin layer chromatography. Pestalotiopsis spp. hasfound to produce novel secondary metabolites with a wide range of bioactivities. Additionally, fluorescence micrographs were supplemented and showed decreasing light intensities emitted by the fluorescent dye ethidium bromide at 605 nm. The interactive 3-D surface plots, which generated using ImageJ 1.52a software, indicated that the extracellular matrix was disrupted. This study concluded that the unknown secondary metabolites from P-03 crude extract have antibiofilm activity against B. cereus s. l. biofilms on stainless steel surfaces and could be an alternative method for biofilm reduction.

Keywords: antibiofilm activity, biofilm reduction, microbiology, secondary metabolites

Edition: Volume 9 Issue 5, May 2020,

Pages: 1074 - 1078