High Performance Thin Layer Chromatography (HPTLC) and High Performance Liquid Chromatography (HPLC) for Qualitative and Quantitative Analysis of Chlorogenic Acid in Calendula Officinalis Linn

Darekar D. P., Sheikh. M. V., Hate. M.S.

Abstract: A simple Chromatographic technique such as HPTLC and HPLC provide valuable additional information to establish the identity of the plant material. The primary goal of this study was to develop a simple and reliable High Performance Liquid Chromatography and High Performance Thin Layer Chromatography method to quantitate polyphenol such as chlorogenic acid present in Calendula Officinalis Linn. using analytical techniques. A combination of this two analytical methods may be useful in quality control analysis as it would give a simple, precise and rapid method.

Keywords: Calendula officinalis, chlorogenic acid, phytoconstituents, polyphenols