A Novel Form of Macrophage Function Inhibition by Leishmania mexicana Infection: Involvement in Inhibition of NF-kB Translocation
Abstract: Manipulation of host cell signalling pathways and pro-inflammatory proteins expression by Leishmania is critical for Leishmanias survival and resultant pathology. Therefore, the characteristics of long time exposure of promastigotes mediated signalling to the MAP kinases, COX-2, iNOS, NO production and NF-B pathways were investigated in comparison to LPS stimulation. Promastigotes caused no effect on phosphorylation of the three MAP kinases. Unlike outcome was observed on COX-2, iNOS and NO production using the same manner. Consequently, expressions of these proteins were also NF-B dependent. Surprisingly, promastigotes inhibit macrophage NF-B translocation into nucleus following LPS stimulation and caused a reversal of promastigotes mediated inhibition of macrophage COX-2, iNOS and NO production. These data demonstrated for the first time the role of NF-B translocation in mediating the effect of L. mexicana promastigotes on pro-inflammatory proteins which these proteins play a crucial role in innate immunity against Leishmania infection.
Keywords: Leishmania mexicana Promastigotes NF-B COX-2 iNOS NO
How to Cite?: Muhannad Shweash, "A Novel Form of Macrophage Function Inhibition by Leishmania mexicana Infection: Involvement in Inhibition of NF-kB Translocation", Volume 3 Issue 10, October 2014, International Journal of Science and Research (IJSR), Pages: 1179-1192, https://www.ijsr.net/getabstract.php?paperid=OCT14215, DOI: https://dx.doi.org/10.21275/OCT14215