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Research Paper | Microbiology | India | Volume 5 Issue 6, June 2016

In-vitro Transit Tolerance of Probiotic Bacillus species in Human Gastrointestinal Tract

Ajitha S. Nair | Aparna V. Dubhashi

Abstract: The present work describes the tolerance of five potential probiotic Bacillus species towards acid and bile in human gastrointestinal tract by in-vitro studies. Artificial gastric and intestinal juice was prepared and adjusted to different pH values for acid and bile tolerance study. A time course analysis up to 4 hours was studied with respect to viability. There was a statistically significant growth response of isolates under high acidic conditions (p < 0.05) as confirmed by ANOVA test. Bacillus VS-5 was found to be the best with 98.78 % survival rate for 3 hours exposure to pH 1.2 and 98.10 % to 2 % bile concentration when exposed for 4 hours. Considering the residence time of food in stomach and intestine, this isolate proves to have an advantage of surviving and also proliferating in the intestinal tract, which is a desirable characteristic of probiotic cultures.

Keywords: Probiotic, Bacillus species, transit tolerance, gastric juice, ANOVA test

Edition: Volume 5 Issue 6, June 2016,

Pages: 1899 - 1902