In vitro Plant Regeneration from the Nodal Explants of Spermacoce articularis L. f.

Biula Preethi C., Velayutham P., Karthi C.

Abstract: An in vitro regeneration of plantlets from the nodal explants of Spermacoce articularis L. f. , a member of Rubiaceae, has been conducted. Nodal regions with axillary buds were cultured on MS medium supplemented with different concentrations of BAP and KIN ranging from 5 to 25 M alone or in combination of both. Among the two cytokinins tested 10 M BAP was found to be the best for shoot proliferation. However, combination of KIN with 10 M BAP further enhanced the rate of shoot proliferation. Maximum number of 31.8 shoots per node with 100 % shoot induction frequency were observed on MS medium containing 10 M BAP and 4 M KIN. The regenerated shoots were cultured on half strength MS medium containing different concentration of IAA, IBA and NAA for root induction. Among these three auxins, 4 M IBA was found to be the best hormone for rooting. The well rooted plantlets were successfully hardened with 80 % survival rate.

Keywords: Spermacoce articularis, Nodal Segments, Direct Regeneration, Cytokinins, Rooting, Auxins

How to Cite?: Biula Preethi C., Velayutham P., Karthi C., "In vitro Plant Regeneration from the Nodal Explants of Spermacoce articularis L. f.", Volume 5 Issue 8, August 2016, International Journal of Science and Research (IJSR), Pages: 226-230, https://www.ijsr.net/getabstract.php?paperid=ART2016824, DOI: https://dx.doi.org/10.21275/ART2016824