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Research Paper | Botany | India | Volume 5 Issue 8, August 2016 | Popularity: 6.1 / 10

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In vitro Plant Regeneration from the Nodal Explants of Spermacoce articularis L. f.

Biula Preethi C., Velayutham P., Karthi C.

Abstract: An in vitro regeneration of plantlets from the nodal explants of Spermacoce articularis L. f. , a member of Rubiaceae, has been conducted. Nodal regions with axillary buds were cultured on MS medium supplemented with different concentrations of BAP and KIN ranging from 5 to 25 M alone or in combination of both. Among the two cytokinins tested 10 M BAP was found to be the best for shoot proliferation. However, combination of KIN with 10 M BAP further enhanced the rate of shoot proliferation. Maximum number of 31.8 shoots per node with 100 % shoot induction frequency were observed on MS medium containing 10 M BAP and 4 M KIN. The regenerated shoots were cultured on half strength MS medium containing different concentration of IAA, IBA and NAA for root induction. Among these three auxins, 4 M IBA was found to be the best hormone for rooting. The well rooted plantlets were successfully hardened with 80 % survival rate.

Keywords: Spermacoce articularis, Nodal Segments, Direct Regeneration, Cytokinins, Rooting, Auxins

Edition: Volume 5 Issue 8, August 2016

Pages: 226 - 230

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