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Research Paper | Microbiology | India | Volume 3 Issue 6, June 2014

Isolation; Biochemical Characterization and Inoculation Effect of Azospirillum on the Growth of Wheat

Pragya Rathore

Abstract: Azospirillum is a free living plant growth promoting bacteria (PGPB) which affects the growth and yield of crops. Inoculation of azospirillum results in reduction in need for nitrogen fertilization and obvious increase in productivity of the inoculated crop. In the present study the organisms were screened from the crop soils and biochemical characterization was carried out. The effect of the isolated organism was studied on the germination of inoculated wheat. Azospirillum is a PGPB has been known to increase the productivity in terms of grain yield content and the growth of plant. The growth is stimulated because the organism also produces growth hormones like auxin. To study the effect of hormones on seed germination the seeds were inoculated with the organism and were allowed to germinate in 0.8 % agar medium. Two times increase in germinated seed shoot length was observed. Pot culture study on wheat plant was also carried out which demonstrated significant increase in the shoot length; root length; fresh weight and dry weight.

Keywords: Azospirillum, wheat, plant growth, root length, shoot length, microcosms

Edition: Volume 3 Issue 6, June 2014,

Pages: 626 - 628