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Research Paper | Chemistry | India | Volume 3 Issue 6, June 2014 | Rating: 6.7 / 10
A New Spectrophotometric Determination of Endosulfan and Its Application
Kalpana Wani, M. K Rai, Raisa Khatoon
Abstract: A new spectrophotometric method has been developed based on the modification of fujiwara reaction for the determination of endosulfan in sub ppm level. Endosulfan on its alkaline hydrolysis gives chloroform; which can be reacted with pyridine to produce pink colour. In the present method the pink colour obtained in the above reaction is discharged with a few drops of acetic acid followed by the addition of p-aminoazobenzene reagent which gives an orange coloured dye. The absorbance maxima of orange coloured dye formed is measured at 490 nm. Beers law is obeyed over the concentration range of 1- 36 g in a final solution volume of 25ml. The molar absorptivity and Sandells sensitivity were found to be 6.71105 Lmol-1 cm-1 and 0.2210-3g cm-1 respectively. The standard deviation and relative standard deviation for the absorbance value were found to be 0.0148 and 4.05 % respectively. This method is free from interference of other organochlorines pesticides and can be successfully applied for the determination of endosulfan in various environmental samples.
Keywords: spectrophotometry, endosulfan, p-aminoazobenzene reagent, pyridine, samples
Edition: Volume 3 Issue 6, June 2014,
Pages: 202 - 205