A New Spectrophotometric Determination of Endosulfan and Its Application

Kalpana Wani, M. K Rai, Raisa Khatoon

Abstract: A new spectrophotometric method has been developed based on the modification of fujiwara reaction for the determination of endosulfan in sub ppm level. Endosulfan on its alkaline hydrolysis gives chloroform; which can be reacted with pyridine to produce pink colour. In the present method the pink colour obtained in the above reaction is discharged with a few drops of acetic acid followed by the addition of p-aminoazobenzene reagent which gives an orange coloured dye. The absorbance maxima of orange coloured dye formed is measured at 490 nm. Beers law is obeyed over the concentration range of 1- 36 g in a final solution volume of 25ml. The molar absorptivity and Sandells sensitivity were found to be 6.71105 Lmol-1 cm-1 and 0.2210-3g cm-1 respectively. The standard deviation and relative standard deviation for the absorbance value were found to be 0.0148 and 4.05 % respectively. This method is free from interference of other organochlorines pesticides and can be successfully applied for the determination of endosulfan in various environmental samples.

Keywords: spectrophotometry, endosulfan, p-aminoazobenzene reagent, pyridine, samples

How to Cite?: Kalpana Wani, M. K Rai, Raisa Khatoon, "A New Spectrophotometric Determination of Endosulfan and Its Application", Volume 3 Issue 6, June 2014, International Journal of Science and Research (IJSR), Pages: 202-205, https://www.ijsr.net/getabstract.php?paperid=20132099, DOI: https://dx.doi.org/10.21275/20132099