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Research Paper | Microbiology | Iraq | Volume 6 Issue 7, July 2017
Evaluation of Enzyme Cellulase Production and its Activity, Isolated from Local Fungi
Mohammed H. Mushrif | Luai F. Zghair | Muzahim Alkabban
Abstract: Eight Fungal species were isolated from the rhizosphere of wheat Triticum aestivum ( Trichoderma harzianum, T. viride, T. longibrachiatum, Alternaria alternate, Fusarium solan, Aspergillus niger, Rhizoctonia solani and Fusarium oxysporum). All the isolated fungi had the capacity to cellulase enzyme production in solid media (CMC- Agar), moreover, T. viride gave the highest capability for the production of the enzyme (6.14). Rhizoctonia solani gave less productive ratio (3.0). Quantitative test using Mandel liquid media showed that the fungus T. viride had the highest activity of cellulase enzyme (4.39 U/ml), however this fungus had the greatest biomass (8.96 g/L). The fungus Rhizoctonia solani gave lower enzyme activity (2.04 U/ml) and its biomass reached to (4.65 g/L). Studying of different carbon sources for the fungus T. viride showed that sucrose was the best carbon source used with the liquid media for cellulase activity (3.87 U/ml), and the biomass (2.83 g/L). From these findings, such fungi able to produce enzymes when activated depending on the substrate and cultured media.
Keywords: Fungi, cellulace enzyme activity
Edition: Volume 6 Issue 7, July 2017,
Pages: 1373 - 1377
Similar Articles with Keyword 'Fungi'
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Research Paper, Microbiology, India, Volume 11 Issue 1, January 2022
Pages: 723 - 728Microbial Studies to Evaluate Bio-Deterioration of Oil Painting on Masonite board and it's Conservation
Sanjay Prasad Gupta [2] | Nitin Kumar Maurya
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Research Paper, Microbiology, Libya, Volume 11 Issue 10, October 2022
Pages: 1237 - 1242Isolation of Hortaea werneckii for the First Time, and Fungi Related to Producing Aflatoxins from Livestock Feeds in Ghemins, Libya
Latifa Abdel-Hafid Jwieli [5] | Hamzah Khalleefah Naji Abd Allah | Khalid Ali Al-Mabrouk Masoud | Hameda Ali Mohamed Naser El-Moghrabi [3] | Nadia Ali Elramli [4]