Evaluation of Enzyme Cellulase Production and it's Activity, Isolated from Local Fungi
International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
www.ijsr.net | Open Access | Fully Refereed | Peer Reviewed International Journal

ISSN: 2319-7064



Research Paper | Microbiology | Iraq | Volume 6 Issue 7, July 2017

Evaluation of Enzyme Cellulase Production and it's Activity, Isolated from Local Fungi

Mohammed H. Mushrif, Luai F. Zghair, Muzahim Alkabban

Eight Fungal species were isolated from the rhizosphere of wheat Triticum aestivum ( Trichoderma harzianum, T.viride, T.longibrachiatum, Alternaria alternate, Fusarium solan, Aspergillus niger, Rhizoctonia solani and Fusarium oxysporum). All the isolated fungi had the capacity to cellulase enzyme production in solid media (CMC- Agar), moreover, T.viride gave the highest capability for the production of the enzyme (6.14). Rhizoctonia solani gave less productive ratio (3.0). Quantitative test using Mandel liquid media showed that the fungus T.viride had the highest activity of cellulase enzyme (4.39 U/ml), however this fungus had the greatest biomass (8.96 g/L). The fungus Rhizoctonia solani gave lower enzyme activity (2.04 U/ml) and its biomass reached to (4.65 g/L). Studying of different carbon sources for the fungus T. viride showed that sucrose was the best carbon source used with the liquid media for cellulase activity (3.87 U/ml), and the biomass (2.83 g/L). From these findings, such fungi able to produce enzymes when activated depending on the substrate and cultured media.

Keywords: Fungi, cellulace enzyme activity

Edition: Volume 6 Issue 7, July 2017

Pages: 1373 - 1377

Share this Article

How to Cite this Article?

Mohammed H. Mushrif, Luai F. Zghair, Muzahim Alkabban, "Evaluation of Enzyme Cellulase Production and it's Activity, Isolated from Local Fungi", International Journal of Science and Research (IJSR), https://www.ijsr.net/search_index_results_paperid.php?id=ART20174502, Volume 6 Issue 7, July 2017, 1373 - 1377

65 PDF Views | 37 PDF Downloads

Download Article PDF



Similar Articles with Keyword 'Fungi'

Research Paper, Microbiology, Iraq, Volume 6 Issue 7, July 2017

Pages: 1373 - 1377

Evaluation of Enzyme Cellulase Production and it's Activity, Isolated from Local Fungi

Mohammed H. Mushrif, Luai F. Zghair, Muzahim Alkabban

Share this Article

Research Paper, Microbiology, India, Volume 3 Issue 4, April 2014

Pages: 648 - 652

Microbiological Pollution of Air in Lal Bagh Botanical Gardens, Bangalore, Karnataka, India

Nandini.N, Sivasakthivel S

Share this Article

Research Paper, Microbiology, Nigeria, Volume 6 Issue 12, December 2017

Pages: 1484 - 1492

Phytochemical Determination and In Vitro Antimicrobial Activity of Crude Ethanolic Extract of Stem Bark of Boswellia dalzielii

Baha'uddeen Salisu Dandashire; Abdulkadir Magaji Magashi; Bashir Abdulkadir

Share this Article

Research Paper, Microbiology, India, Volume 3 Issue 7, July 2014

Pages: 1713 - 1717

Nodular Micro-Endosymbiontic and Endomycorrhizal Colonization in Elaeagnus latifolia L.: A noble host of Symbiotic Actinorhizal and Endomycorrhizal Association

Liza Handique, Vipin Parkash

Share this Article

Research Paper, Microbiology, India, Volume 4 Issue 9, September 2015

Pages: 1227 - 1232

Impact of Hg (0) on Microbial functional diversity with their Enzyme Profile (MerA)

Rashmita Patel, Ratna Trivedi

Share this Article
Top