Genetic Aspect of Iraqi PregnantWomen with Pre-Eclampsia

Salih M. Al-Khafaji, Anwar M. Al-janabi, BasimaAl-ghzaly, Shehab A.Faris

Abstract: Introduction One of serious problems of pregnancy complication is the Pre-eclampsia syndrome (PE) which mostly leads to increase morbidity and mortality in mother and fetus. Objective we aimed to study and evaluate the genetic polymorphism contribution of MTHFR gene in preeclampsia patients and healthy pregnant women. Methods We examined maternal age, gestational age, BMI, serum homocysteine concentration, proteinuria, systolic blood pressure, diastolic blood pressure and MTHFR (C677T) gene polymorphism in 52 PE and 50 healthy pregnant women. After DNA extraction from blood samples of all participants the PCR-RFLP technique were applied for detection of MTHFR gene polymorphism. Results We found the means of serum homocysteine concentration, proteinuria, systolic blood pressure, diastolic blood pressure which were significantly higher in PE patients compared to healthy control (P0.05). The miner allele frequency of T genotype was significantly higher in PE patients than the subjects of control groups (P0.0001) and the risk of PE was higher by 20.9 folds in homozygous allele genotype (TT) when compared with wild genotype (CC) ( OR 20.9, 95 % CI= 4.06- 107.4 P= 0.003). Conclusion We concluded that the TT genotype of MTHFR gene is increased the risk of PE in pregnant patients of Iraqi population.

Keywords: MTHFR, Pre-eclampsia, Polymorphism, SNP, Iraq