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Research Paper | Biochemistry Science | India | Volume 4 Issue 6, June 2015
GC-MS Analysis of Phytocomponents in the Methanolic Extract of Shorea Robusta
Mathavi P | Nethaji S | Velavan [3]
Abstract: Plants have been an important source of medicine with qualities for thousands of years. Phytochemicals are the chemicals extracted from plants. These organic chemicals are classified as primary or secondary constituents, depending on their role in plant metabolism. GC-MS method used for the analysis of the obtained extract can be an interesting tool for testing the amount of some active principles in herbs used in various industries. The aim of this study was to carry out for identification of bioactive compounds from the whole plant methanolic extract of Shorea robusta by Gas chromatography and Mass spectroscopy (GC-MS). GCMS analysis of methanolic extract was done by standard protocol using the equipment Perkin-Elmer Gas ChromatographyMass Spectrometry, while the mass spectra of the compounds found in the extract was matched with the National Institute of Standards and Technology (NIST) library. The GC-MS analysis revealed the presence of various compounds like 9, 12-Octadecadienoic acid (Z, Z) -, Tetra decanoic acid $$ myristic acid, Nonanedioic acid, dibutyl ester $$ Azelaic acid, 1- (+) -Ascorbic acid 2, 6-dihexadecanoate, Heptadecanoic acid, Phytol isomer, Oleic acid $$ 9-octadecenoic acid (Z), Octadecanoic acid $$ stearic acid, Cis-11, 14-Eicosadienoic acid, methyl ester and Hexadecanoic acid in the methanolic extract of Shorea robusta. These findings support the traditional use of Shorea robusta in various disorders.
Keywords: Gas chromatography and Mass spectroscopy, Shorea robusta, Phytochemistry
Edition: Volume 4 Issue 6, June 2015,
Pages: 1935 - 1938