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M.Tech / M.E / PhD Thesis | Biological Engineering | India | Volume 4 Issue 6, June 2015
Phytochemical Extraction and Characterization of the Leaves of Aloe vera barbadensis for its Anti-Bacterial and Anti-Oxidant Activity
Abstract: The aim of the study was to investigate the phytochemical compounds, anti-bacterial and anti-oxidant activity of different extracts of Aloe vera. The phytochemical compounds were screened by qualitative analysis method. The detected phytochemical constituents were Tannins, Saponins, Alkaloids, Phenols, Terpenoids, Flavonoids. The different solvents such as methanol, petroleum ether, chloroform and aqueous were used to extract the bioactive compounds from the leaves of Aloe vera to screen the anti-bacterial and anti-oxidant activity. The anti-bacterial activity were demonstrated against the bacterial strains like Bacillus subtilis, Bacillus cereus, Staphylococcus aureus and Escherichia coli by disc diffusion method. Methanolic extract showed maximum inhibitory action against the bacterial strains followed by the aqueous extract. The anti-bacterial activity is attributed to anthraquinones and saponins. The anti-oxidant activity was evaluated by DPPH radical scavenging method. The highest radical scavenging activity was of methanolic extract followed by petroleum ether and aqueous extract.
Keywords: Aloe vera, Phytochemicals, Anti-Bacterial, Anti-Oxidant, DPPH, Disc Diffusion
Edition: Volume 4 Issue 6, June 2015,
Pages: 658 - 661