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Research Paper | Entomology | Egypt | Volume 4 Issue 5, May 2015

Isolation and Characterization of Internal Bacteria from the Mosquito, Culexpipiens from Egypt

Fatma H. Galal ^[2] | Amany Abu Elnasr | Ibtesam Abdallah | Alaa Eddeen M. Seufi | Omaima Zaki

Abstract: A total of 11 bacterial isolates were isolated from internal body of the mosquito, Culex pipiens (eggs, 4th larval instars, pupae, adult males and adult females) in one gonotrophic cycle. Two bacterial isolates were Gram +ve stained (Staphyllococcus epidermidis and Bacillus subtilis) and the other nine bacterial isolates were Gram -ve stained. Phenotypic, biochemical biotyping and differential media were used to characterize and identify our isolate. These methods could differentiate our isolates to species level. In addition, many of the obtained strains were proved to have economic and commercial importance. Bacterial antagonism was studied and it was noticed that B. subtilis was antagonistic to both E. coli and S. aureus, while S. flexneri was antagonistic to S. typhimurium, and S. epidermidis was antagonistic to the multi-drug resistant strain, S. aureus.

Keywords: Culex pipiens, bacterial isolates, bacterial antagonism

Edition: Volume 4 Issue 5, May 2015,

Pages: 2682 - 2688

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