Quantitative Estimation of Some Phytochemical and Determination of Metalic Elements from Pueraria tuberosa (Roxb. ex Willd.) DC. Tuber

K. B. Theng, A. N. Korpenwar

Abstract: In present study quantitative estimation of some phytochemicals and metalic elements were carried out. Quantitative estimation of P. tuberosa tuber was performed to identify the constituents as total alkaloids, total flavonoids, total saponin and total terpenoid by standard method. Quantitative estimation was shown that P. tuberosa tuber powder contained alkaloid 9.84 %, flavonoids 4.23 %, terpenoids 1.32 % and saponin 4.00 %. The ICP-AES analysis of ethanolic soxhlet extracted powdered of P. tuberosa tuber was showed the presence of total 22 elements such as Al, B, Ba, Be, Ca, Co, Cr, Cu, Fe, Hf, K, Mg, Mn, Na, Ni, P, S, Sc, V, Y, Yb and Zr. Quantitative analysis of six major elements was revealed their concentration as Fe= 0.37ppm, Mg= 0.4ppm, Al= 1.56ppm, K= 1.1ppm, Ca= 1.52 ppm and Cr= 0.0071ppm. The elements obtained during XRD analysis were also showed similarity with ICP-AES result. The result was showed that P. tuberosa tuber contain various medicinally important phytoconstituents and metalic elements. Hence this study revealed that the further studies are necessary for identification, isolation and purification of biologically active compounds and various elements.

Keywords: Phytochemical, Pueraria tuberosa, ICP-AES spectrometry, X-Ray Diffraction