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Original Research | Biotechnology | Volume 15 Issue 6, June 2026 | Pages: 971 - 986 | India
Green Synthesis of Ag & Cu Nanoparticles from Waterlily (Nymphaea spp.) Plant: Characterization and their Biotechnological Applications
Abstract: This study investigates the effects of green synthesized copper nanoparticles (CuNPs) and silver nanoparticles (AgNPs) on the secondary metabolites of the herb Bacopa monnieri. Nanoparticles were synthesized using waterlily leaf and characterized via XRD, FTIR, DLS, UV-visible spectrophotometry and SEM, revealing spherical particles with diameters between 20 nm and 1 ?m. Experimentation with Murashige and Skoog medium showed that thidiazuron (TDZ) at 1.0 mg L-1 was most effective, yielding 8 shoots per explant. The combination of TDZ with AgNPs at 20 mg L-1 significantly increased shoot production to 10 shoots per explant. Analysis of methanolic extracts indicated a concentration-dependent rise in secondary metabolites, with an optimal range of 20-40 mg L-1, before reaching toxic levels at 50 mg L-1. Reactive oxygen species (ROS) indicators and antioxidant enzyme activity increased, suggesting that sub-toxic concentrations of CuNPs and AgNPs enhance Bacopa monnieris secondary metabolism and antioxidant capacity.
Keywords: Green synthesis, Silver nanoparticles, Copper nanoparticles, Waterlily, Bacopa monnieri, Secondary metabolites, Reactive oxygen species, Nanobiotechnology
How to Cite?: Praveen Pathak, Dr Sandeep Kumar Verma, "Green Synthesis of Ag & Cu Nanoparticles from Waterlily (Nymphaea spp.) Plant: Characterization and their Biotechnological Applications", Volume 15 Issue 6, June 2026, International Journal of Science and Research (IJSR), Pages: 971-986, https://www.ijsr.net/getabstract.php?paperid=SR26618114806, DOI: https://dx.doi.org/10.21275/SR26618114806