Method Validation and Kinetic Investigation of Ciprofloxacin Mutarotation by Polarimetry

Lalasaheb Kashid

Abstract: A reliable and rapid polarimetric method was developed for the estimation of Ciprofloxacin Hydrochloride in bulk and pharmaceutical dosage forms. Standard and sample solutions were prepared in distilled water, and the method was validated according to ICH guidelines. Linearity was demonstrated in the range of 0.4?4.0 mg/mL with a correlation coefficient (r?) of 0.9930. The accuracy of the method was confirmed with a mean recovery of 99.56???0.28%. Precision studies showed %RSD values below 2% for both intra- and inter-day measurements, indicating excellent reproducibility. The method showed good robustness with minimal variation in optical rotation due to sample positioning. Sensitivity was established with LOD and LOQ values of 0.2795 mg/mL and 0.8472 mg/mL, respectively. The validated method was successfully applied to the kinetic study of ciprofloxacin mutarotation in acidic medium at constant temperature. Overall, the method is simple, accurate, precise, and suitable for routine quality control and kinetic investigations.

Keywords: Ciprofloxacin, Method Validation, Polarimetry, Kinetic Mutation

How to Cite?: Lalasaheb Kashid, "Method Validation and Kinetic Investigation of Ciprofloxacin Mutarotation by Polarimetry", Volume 14 Issue 6, June 2025, International Journal of Science and Research (IJSR), Pages: 789-795, https://www.ijsr.net/getabstract.php?paperid=SR25611134300, DOI: https://dx.doi.org/10.21275/SR25611134300