Cytotoxic Activity of Sauromatum venousm (Ait.) Kunth. Against Carcinoma Cell Line

M. U. Ghurde, S. N. Malode, R. R. Godse, P. A. Wadegaokar

Abstract: In the present study, human pancreatic cancer cell lines (MiaPaca - 2) were used to assess the cytotoxic effect of Sauromatum venosum corm extract. The MTT assay was used to measure the cytotoxic activity on human pancreatic cells. A calorimetric approach based on the ability of metabolically active cells to cleave the yellow tetrazolium salt MTT to an insoluble purple formazan crystal by the mitochondrial enzyme succinate dehydrogenase was used to quantify cell proliferation following a 48 - hour treatment with the extracts. The soluble formazan dye in DMSO was directly quantified by scanning multiwel spectrophotometer. The findings demonstrated that the corm`s acetone extract significantly inhibited the cell growth in dose dependent manner. It exhibited that the extract had a potent cytotoxic action against the MIAPaCa - 2cells with an IC50 value is 92.69?g/ml. The findings revealed that the high concentration of alkaloids, saponins, and polyphenolic chemicals in the corm acetone extract may be the cause of its cytotoxic action. Therefore, it can be concluded that Sauromatum venosum corm may kill MIAPaCa - 2 cancer cells making it a potentially effective therapeutic agent for the treatment of pancreatic cancer.

Keywords: Sauromatum venosum corm, acetone extract, cytotoxicity, MTT assay, 5 - Flurouracil