Isolation of L-Asparaginase Enzyme from Microbes

Abhishek Ashok Nagergoje

Abstract: L-Asparaginase (EC 3.5.1.1) is a crucial enzyme with significant therapeutic applications, especially in the treatment of acute lymphoblastic leukemia (ALL). It catalyzes the hydrolysis of L-asparagine into L-aspartate and ammonia, thus depleting the supply of asparagine, which is essential for the survival of certain tumor cells. L-Asparaginase is produced by various microorganisms, including bacteria, fungi, and actinomycetes. The microbial production of this enzyme has drawn attention due to its cost-effectiveness, ease of fermentation, and ability to produce high yields. This review article focuses on the microbial sources of L-Asparaginase, methods of isolation and purification, and the importance of optimizing the production conditions for industrial and therapeutic applications.

Keywords: L-Asparaginase, e-coli, Aspartic acid

How to Cite?: Abhishek Ashok Nagergoje, "Isolation of L-Asparaginase Enzyme from Microbes", Volume 13 Issue 9, September 2024, International Journal of Science and Research (IJSR), Pages: 1576-1577, https://www.ijsr.net/getabstract.php?paperid=SR24926112010, DOI: https://dx.doi.org/10.21275/SR24926112010