Effect of Temperature and pH on Lipase Production Activity of Fungi

Dhiraj Kumar, Dr. Md. Anzer Alam

Abstract: Fungal lipases remain preferable than lipases obtained from other sources due to their low cost of extraction as well as thermal and pH stability. Best incubation temperature for lipase enzyme activity in Penicillium chryogenum and Aspergillus oryzae it remain as 40oC while in Fusarium solani it remain as 30oC. All three tested fungal species showed different peaks with regard to impact of pH on lipase activity. In Pencicillium chrysogenum and Aspergillus oryzae optimum enzyme activity has been observed from pH 5.0 to 7.0 and 6.0 to 8.0 respectively. Whereas in Fusarium solani it has been observed from pH 4.0 to 9.0 It was observed during this research study that, besides temperature, pH also has a great influence on the characterization of enzyme. The optimum initial pH for lipase activity was maintained as 7.0 in all fungal strains tested.

Keywords: Temperature, pH, Lipase production, Fungi