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Research Paper | Biochemistry | India | Volume 13 Issue 11, November 2024 | Popularity: 5.2 / 10

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Identification of Protein Engineering Strategies of Insulin Receptor Tyrosine Kinase - A Protein by Using Hotspot Wizard Webserver

Kaushick Mishra, Soumendra Nath Talapatra

Abstract: Insulin receptor (IR) tyrosine kinase (Tyr - K) A is well - known larger, multi - sphere and integral membrane protein. The well - established metabolic abnormality is the insulin resistance in which patients are found with non - insulin - dependent diabetes mellitus. The objective of the present study was to identify the protein engineering strategy as per hot spots for protein stability and site - specific mutation of IR - Tyr - K A protein by using an online tool, HotSpot Wizard (version, 3.1). The prediction results through an output interface where functional hot spots, stability hot spots (structural flexibility), correlated hot spots and stability hot spots (sequence consensus) recorded. Moreover, average B - factor along with mutability rate and score were also obtained. In conclusion, pocket identification and mutability prediction of IR - Tyr - K A could be led to detect structural alternation mainly in insulin resistance and space for ligand binding pocket in new drug discovery for disease prevention. This prediction is suggested to compare with experimental hotspots for IR - Tyr - K A protein as per disease therapy after new drug design.

Keywords: Drug design, Hotspots design, Insulin receptor (IR) tyrosine kinase, Protein engineering strategies, Site directed mutagenesis

Edition: Volume 13 Issue 11, November 2024

Pages: 153 - 158

DOI: https://www.doi.org/10.21275/SR241101123703

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