Green Synthesis and Characterization of Nano Silver Doped Nano Hydroxyapatite (nAg-nHAP) using OcimumTenuiflorum Leaf Extract and Sea Shell - A New Approach

M Sundarrajan, S Durgadevi, V Santhanam

Abstract: In this work Calcium Oxide (CaO), a prime material for hydroxyapatite (HAP) synthesis, was derived from sea shells by calcination at 1200?C for 2 hours . The calcinedCaO was treated with phosphoric acid with ammonium buffer (pH 10) and Nanohydroxyapatite (nHAP) was synthesized. The colloidal dopant solution of nanosilver was green synthesized fromsilver nitrate solution using ocimumtenuiflorum leaf extract. Colloidal nanosilver (nAg) was added to nHAP and the obtained nanosilver doped nano hydroxyapatite (nAg-nHAP) was calcined at 700 ?C for 2 hours. The XRD results of prepared nAg-nHAP powder showed essential peaks of standard nHAP JCPDS file No. 09-0432. The Ca/P molar ratio of synthesised nAg-nHAP observed as 1.71, showed a minimum deviation from standard nHAP due lattice disturbance on nAg doping.The nAg-nHAPnanoparticles synthesized in the range 22-44.2 nm. The FTIR, UV-Vis, XRD, SEM and EDAX analysessubstantiated the confirmation of hydroxyapatite structure with the incorporation of nanosilver(nAg) along with calcium(Ca), phosphorous(P) and oxygen(O).

Keywords: Silver nanoparticles; nano hydroxyapatite; nano silver doped nano hydroxyapatite; sea shells; ocimumtenuiflorum leaf extract