International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
Call for Papers | Fully Refereed | Open Access | Double Blind Peer Reviewed

ISSN: 2319-7064


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Experimental Result Paper | Dentistry | India | Volume 12 Issue 9, September 2023


Effectiveness of Natural Irrigants Activated By Diode Laser on Elimination of E. Faecalis Biofilms- An in Vitro CLSM Study

Dr. Krishna Prasada L | Dr. Banashree Bhagawati


Abstract: Disinfecting the root canal is a crucial paradigm in the maintenance of teeth which have undergone root canal therapy. Though, a number of antibiotics and surfactants are being widely used in the treatment of biofilms however, the current trend is towards identification of natural products in disinfection. Aim: To investigate the impact of naturally occurring irrigants, activated by diode laser, on mature biofilms of Enterococcus faecalis in vitro. Materials & Methods: Thirty-six premolar teeth, each with a single root, underwent decoronation followed by determination of working length. The root canals were prepared utilizing Pro-Taper Gold rotary nickel titanium instruments and irrigated with 3% sodium hypochlorite and 17% EDTA solutions. Subsequently, the teeth were vertically sectioned along the midsagittal plane into two halves. The resulting prepared teeth were randomly assigned to one of six groups, each containing six teeth, and treated with laser activation. These groups included: Group 1 - 0.2% Chitosan, Group 2 - Neem Extract, Group 3 - Curcumin Extract, Group 4 - 0.2% Chitosan + Neem Extract (1:1 ratio), Group 5 - 0.2% Chitosan + Curcumin Extract (1:1 ratio), and Group 6, which served as the control and was treated with saline. The dentin specimens were then examined carefully, with random areas of the biofilm observed utilizing a confocal laser scanning microscope. Statistical Analysis: The results were subjected to one ? way ANOVA followed by Bonferri test. Results: All groups had a significantly higher percentage of dead bacteria than the saline control. Neem Extract had a significantly higher percentage of dead bacteria than other groups. Conclusion: Within the limitations of this in vitro study it can be concluded that Neem extract has superior antibiofilm activity against the E.faecalis and there was no significant difference for the bacterial kill efficacy between activated and non-activated neem extract.


Keywords: Enterococcus faecalis, Neem, Chitosan, irrigants, Confocal, diode laser


Edition: Volume 12 Issue 9, September 2023,


Pages: 1528 - 1532


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