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Case Studies | Molecular Biology | India | Volume 12 Issue 9, September 2023

Advancements in Molecular Diagnostics: A Comprehensive Multiplex RT-PCR Approach for Detecting BCR-ABL Fusion Transcripts in Hematological Disorders

Nelson Amirtharaj | Ananthvikas Jayaram | Sripriya S

Abstract: The Philadelphia chromosome was the first to be discovered as a chromosomal abnormality in haematological illnesses. The recommended method for detecting BCR-ABL in CML and ALL is RT-PCR. The three most commonly identified mRNA transcripts are e1a2 in ALL, e13a2 and e14a2 in CML and ALL. It can detect fusion genes in preserved cell pellets from individuals expressing any subtype of the major bcr (M-bcr) e13a2, e14a2, e13a3 and e14a3, the minor bcr (m-bcr) e1a2 and e1a3, the micro bcr (?-bcr) e19a2 and e19a3, and the nano bcr (n-bcr) e6a2 and e6a3. The Multiplexed Real Time Polymerase Chain Reaction (RT-PCR) assay was used for testing. Agarose gel electrophoresis was used to examine the major (e14a2, e13a2) and minor (e1a2) transcripts. The outcomes were anticipated using known samples of internal control, as well as positive and negative controls. The Multiplex RT-PCR assay is a clinically useful, efficient, and quick method for detecting BCR-ABL fusion transcripts. We report a new, quick, and dependable multiplex PCR method for detecting typical and atypical BCR-ABL transcripts.

Keywords: BCR-ABL fusion proteins, Chronic myeloid leukemia, Acute lymphoblastic leukemia, Multiplex-PCR. Leukemia

Edition: Volume 12 Issue 9, September 2023,

Pages: 989 - 994