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Research Paper | Pharmacology and Toxicology | Volume 12 Issue 9, September 2023 | Pages: 553 - 559 | India
Exploring the Link between Type 2 Diabetes Mellitus and Triple Negative Breast Cancer: Insights from mRNA Expression Analysis in Tertiary Care Hospital
Abstract: Background: Type 2 diabetes mellitus is associated with hyperinsulinemia, hyperglycaemia, adipokine changes (increased leptin and decreased adiponectin), chronic low-grade inflammation (increased interleukin-6 (IL-6), tumour necrosis factor-alpha (TNF-), and interferon ?). (INF-), increased levels of oxidative stress. These T2DM-related factors are thought to enhance tumour growth by engaging in signalling pathways involved in cell proliferation, migration, angiogenesis, inflammation, invasion, and apoptosis. However, the mechanisms of association between T2DM and breast cancer are still not fully understood. Aims/Objectives: Toanalyze the evaluation of role of diabetes mellitus related mRNA in triple negative breast cancer population of Bihar, whether the presence of diabetes in breast cancer patients led to a different mRNA expression before and after chemotherapy. Materials/Materials: Breast cancer and aged matched healthy control blood sample were taken in Paxgene tube. Total RNA was extracted from the breast cancer and healthy controls using PAX-gene Blood RNA Kit (Qiagen). ncRNAs and mRNAs were sequenced using the high-throughput and high-sensitivity Novaseq 6000 sequencing platform (Illumina, Inc.). Fast-QC version 0.11.8 was used to evaluate the quality of the pre-processed data. Gene expression was quantified via reads per kilo-base per million mapped reads, which was calculated using the DESeq2 package in R. Normalization of the read counts was performed and the differentially expressed genes for the treatment groups are filtered according to log2FC values (>0) and P-value
Keywords: Breast Cancer, Type 2 Diabetes Mellitus, mRNA, Gene Expression
How to Cite?: Khushboo, Dr. Manish Kumar, Dr. Saajid Hameed, Suchitra Kumari, Dr. Harihar Dikshit, "Exploring the Link between Type 2 Diabetes Mellitus and Triple Negative Breast Cancer: Insights from mRNA Expression Analysis in Tertiary Care Hospital", Volume 12 Issue 9, September 2023, International Journal of Science and Research (IJSR), Pages: 553-559, https://www.ijsr.net/getabstract.php?paperid=SR23905131123, DOI: https://dx.doi.org/10.21275/SR23905131123