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Analysis Study Research Paper | Bio-Organic Chemistry | India | Volume 12 Issue 8, August 2023

Rapid Differentiation of Tea through High - Performance Liquid Chromatography and Chemometric Analysis

Diptiman Das

Abstract: A simple and fast high performance liquid chromatographic method for caffeine using an ODS column and a 50: 50 methanol: water mobile phase system was developed. Caffeine were separated by an methanol gradient within 30 min. The detection limit of the method was approximately 10 mg for all the compounds (by injecting 10 microL). Several green, black and instant teas were analysed using this method. By using the studied compounds as chemical descriptors, linear discriminant analysis was performed and complete differentiation of the green, black and instant teas was achieved.

Keywords: chromatographic method, caffeine, ODS column, methanol - water mobile phase, separation, detection limit, tea analysis, chemometric analysis

Edition: Volume 12 Issue 8, August 2023,

Pages: 2461 - 2464