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Experimental Result Paper | Microbiology | India | Volume 12 Issue 3, March 2023

Biofilm Formation of Stenotrophomonas Maltophilia and the Effect of Antibiotics on Biofilm Formation in vitro

Sona P Hydrose ^[2] | Rasmi T R

Abstract: Nosocomial infections, which are also commonly referred to as healthcare-related diseases, are a considerable burden on hospital and patient administration around the world. Regular hospital admissions of 10% of the population result in millions of deaths that could have been readily prevented. (CDC, 2020). About 1- 3% of deaths worldwide are caused by nosocomial illnesses (CDC, 2020). The most highly developed nations, such as the USA, have recorded about 2 million nosocomial infection cases, of which 70% were thought to be caused by the medical equipment used during the course of the treatment. The term "biofilm" refers to microbial communities that adhere to surfaces. The aggregation of microorganisms on a surface and their ability to create antibiotic resistance are the principal problems of concern. In this research, biofilm was discovered using the microtitre plate method using 15 isolates of S. maltophilia from different clinical samples. The antimicrobial agents tested against biofilm formation by S.maltophilia included Levofloxacin and Ticarcillin clavulanic acid. MIC is detected by microbroth dilution method. Each drug was tested at one-half, one-fourth, and one-eighth the MIC to study its effect on S. maltophilia biofilm formation. All isolate were found to be biofilm formers by microtitre plate assay. Sub inhibitory concentration of Levofloxacin and Ticarcillin clavulanic acid reduces biofilm production of S. maltophilia.

Keywords: Biofilm formation, Nosocomial infection, Stenotrophomonas maltophilia, Minimum inhibitory concentration

Edition: Volume 12 Issue 3, March 2023,

Pages: 1687 - 1691