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Review Papers | Pathology | India | Volume 12 Issue 3, March 2023
Use of Immature Reticulocyte Fraction in Clinical Settings
Abstract: IRF is an indicator of erythropoiesis used by automated analyzers. The manual reticulocyte count includes immature and mature reticulocytes while counting all RNA stained cells, takes a lot of time and effort. Reticulocyte analysis by flow cytometry is more accurate and sensitive. The measurement of fluorescence intensity enables the measurement of reticulocyte maturity. IRF provides an understanding of erythropoietic activity in the bone marrow and how it responds to therapy. It is a straight forward, efficient, affordable, repeatable, and trustworthy parameter. It is critical for the assessment of aplastic anaemia in pancytopenia, acute leukaemia patients', post-chemotherapy bone marrow recovery, and for directing stem cell harvest in autologous peripheral blood stem cell transplant. To compare the results from various analyzers, it is essential to get an international agreement on the definition and reference range of IRF. The data was gathered from Google Scholar that was published in various scientific journals. The clinical utility of IRF was evaluated systematically in the research publications. The benefits, drawbacks, and applications of IRF are determined based on review. Numerous studies with promising findings have been evaluated. There is an urgent need for the normal value standardization. Further research in the area will aid to overcoming current difficulties.
Keywords: Immature reticulocyte fraction, IRF, High-fluorescence reticulocytes, HRF, Medium-fluorescence reticulocytes, MRF, Low-fluorescence reticulocytes, LRF, Reticulocyte haemoglobincontent, CHr
Edition: Volume 12 Issue 3, March 2023,
Pages: 1309 - 1313