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Research Paper | Botany | Nigeria | Volume 12 Issue 2, February 2023

Antibacterial Activity of Chlorine Disinfectant against Potential Biofilms Forming Bacteria

Oyelakin A.O. | Olajolumo S.J. | Sunday T.V. | Fadahunsi D.R.

Abstract: Chlorination of bacteria by active chlorine compounds with the aim of killing them occurs both in a variety of disinfection processes. The research was carried out to determine the antibacterial activity of chlorine disinfectant against potential biofilms forming bacteria. The samples were collected from Oluyole Tap, Mark Mercy Tap and Mark Mercy Sink. Standard analytical method was used to determine the parameters. The result showed that the bacterial contaminant was Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus cereus. The result also showed that Pseudomonasaeruginosa from Mark Mercy Tap had the highest zone of inhibition 34.0mm at 25% concentration of chlorine disinfectant. At 75%, concentration Pseudomonasaeruginosa had 20.0mm zone of inhibition, at 50% concentration 13.0mm zone of inhibition and at 100% concentration 11.0mm zone of inhibition. In Staphylococcusaureus from Oluyole Tap, the highest inhibition concentration is 28.0mm zone of inhibition at 50% concentration followed by 24.0mm zone of inhibition at 100% concentration and 22.0mm zone of inhibition at 75% concentration. While Bacilluscereus form Mark Mercy sink had the highest inhibition concentration of 22.0mm zone of inhibition at 75% concentration, followed by 18.0mm zone of inhibition at 50% concentration and 14.0mm zone of inhibition at 100% concentration while there is no inhibition at 25%. Antimicrobial activity for Staphylococcusaureus, Pseudomonasaeruginosa and Bacilluscereus was done by 50% of chlorine which was found to be effective in all organisms. It was concluded that the disinfectant solution are effective for 27 to 37 days if stored in screw cap bottles, they have the potential to be used in the dental setting as a surface disinfectant and a sterilant for semi-critical heat sensitive instruments.

Keywords: Antibacterial, Biofilms, Chlorine, bacteria, concentration

Edition: Volume 12 Issue 2, February 2023,

Pages: 1022 - 1024