Retroprospective Study of Microbiological Profile and Antimicrobial Sensitivity Pattern in Chronic Otitis Media (Squamous Type) in and Around Prayagraj Uttar Pradesh

Abstract: Aim: Isolate and identify the common microorganism causing chronic otitis media (squamous type). Determine the antimicrobial sensitivity pattern in COM (squamous type) in Prayagraj and surrounding areas. Material & Methods: Total number of samples (n) in our study diagnosed as COM (Squamous type) were 170. Data of 100 samples were collected from retrospective study and 70 samples from prospective study. The specimen for microbiological study was taken from the middle ear cavity and mastoid antrum (under planned OT after meatotomy and antrostomy respectively) in the form of two ear swab under sterile conditions and transferred immediately for staining, culture and sensitivity testing. Both swabs were processed for direct smear examination for Gram staining, AFB staining, culture (under inoculation in Mac Conkey agar, blood agar plate and chocolate agar). and sensitivity testing (Kirby Bauer disc diffusion method). Organism isolated was identified by standard microbiological methods and antimicrobial sensitivity pattern was obtained for further analysis. Results: Chronic otitis media (COM) is an important global public health problem. Monomicrobial etiology, especially Staphylococcus aureus and Pseudomonas species was found most common in our study. Staphylococcus aureus was most sensitive to cefoperazone + sulbactam (56.92%), followed by ceftriaxone and highly resistance to amoxyclav. Pseudomonas was most sensitive to piperacillin+tazobactam.

Keywords: Staphylococcus aureus, Pseudomonas, cefoperazone + sulbactam, sensitive, resistance

Edition: Volume 12 Issue 11, November 2023,

Pages: 369 - 376